Avian Paramyxovirus (Newcastle Disease Virus) PCR

• Swabs**: nasal, oropharyngeal, tracheal (preferred for poultry), cloacal
• Tissues: lungs, trachea. Tissues can be fresh (preferred) or frozen.
• Culture supernatant
• Chorioallantoic fluid

**Poultry testing is conducted on a per flock basis; the lab may pool up to 5 swab samples. Wild bird and hobby poultry testing is done per bird; no pooling of samples is done.

• Leak-proof primary container for fluids and tissues
• Swabs must be kept moist; use viral transport media or place swab in red top tube with 1-2 ml sterile saline.

• Please provide vaccination history: were birds recently vaccinated against Newcastle Disease Virus (NDV)? If so, what type of vaccine was used?
• Label submission form and primary container “High Risk”.
• Label shipping container “Special Handling”.
• Ship all samples with cold packs.