- CBC
- Collect blood in EDTA (lavender top) tube. Minimum volume is ½ of total tube. Invert tube at least 5 times to mix anticoagulant with blood.
- Refrigerate blood until shipped; place cold packs in shipment. Samples should be shipped overnight Monday-Thursday to arrive at VMDL the day after collection.
- Submit 2 freshly made smears along with the blood.
- Use plastic slide holders rather than cardboard mailers if possible.
- Protect slides from formalin fumes.
- Chemistries
- Minimum volume: 1 ml of serum or plasma for panels, 0.5 ml if requesting 6 or fewer individual tests.
- Collect blood in red top, serum separator, or lithium heparin (green top) tubes. Invert green top tubes at least 5 times to mix anticoagulant with blood.
- Avoid using small gauge needles or forcing blood into collection tubes as this will cause hemolysis.
- Serum (red top or SST) and plasma (green) tubes must be centrifuged. Remove the serum/plasma and transfer to a sterile tube within 1 hour of collection.
- Ship on ice Monday-Thursday to arrive at the VMDL the day after collection.
- Cytology (Aspirates, imprints, smears)
- Submit fluids in EDTA (lavender top) tubes. Direct smear slides must also be submitted at the same time.
- Air-dry smears. Do not fix. Keep as dust-free as possible.
- Bone marrow aspirate slides or bone marrow core biopsies should be accompanied by either current CBC results or fresh blood smears and EDTA blood (lavender top) collected at the same time as the bone marrow sample.
- All fluids should be shipped Monday-Thursday for next day delivery. Place cold packs in shipment.
- Cerebrospinal Fluid (CSF)
- For cell count and slide preparation:
- Collect blood from the patient in a red top or SST tube. Allow to clot, centrifuge, and collect serum.
- To a clean tube with no anticoagulant (red top), add 1 part serum to 9 parts CSF. Example: if you have 0.45 ml of CSF, add 0.05 ml of serum. Label this tube “CSF with Serum”. Do not use all of the CSF for this as you will need some for protein determination.
- For protein determination:
- Label the tube with the remaining CSF “Straight CSF”.
- Ship Monday-Thursday for next day delivery. Place cold packs in shipment.
- For cell count and slide preparation:
- Urinalysis
- Sample size 5 -10 ml shipped in a sterile container.
- Indicate method of collection: cystocentesis, catheter, free catch, etc.
- Ship Monday-Thursday for next day delivery. Place cold packs in shipment.
- Fecals: See Parasitology
- Samples not listed or special requests
- Contact the Clinical Pathology lab at 573-882-3338.