Newcastle Disease Virus PCR

Lab Section	Molecular
Test Description	Polymerase chain reaction (PCR) that looks for the presence of avian paramyxovirus antigen, including PMV-1 (Newcastle Disease Virus)
Species	Avian (domestic and wild)

SUBMISSION DETAILS

Sample Type	Swabs: nasal, oropharyngeal, tracheal (preferred for poultry), cloacal Tissues: lungs, trachea. Tissues can be fresh (preferred) or frozen. Culture supernatant Chorioallantoic fluid Poultry testing is conducted on a per flock basis; the lab may pool up to 5 swab samples. Wild bird and hobby poultry testing is done per bird; no pooling of samples is done.
Container	Leak-proof primary container for fluids and tissues Swabs must be kept moist; use viral transport media or place swab in red top tube with 1-2 ml sterile saline.
Special Submission Instructions	Please provide vaccination history: were birds recently vaccinated against Newcastle Disease Virus (NDV)? If so, what type of vaccine was used? Label submission form and primary container “High Risk”. Label shipping container “Special Handling”. Ship all samples with cold packs.
Turnaround time	1-2 business days

The University of Missouri Veterinary Medical Diagnostic Laboratory (VMDL) is a full-service veterinary diagnostic laboratory and Missouri’s only laboratory accredited by the American Association of Veterinary Laboratory Diagnosticians. The VMDL is a Level I Lab of the National Animal Health Laboratory Network, Tier I Lab of the FDA Veterinary Laboratory Response Network, and a National Poultry Improvement Plan authorized lab. The VMDL maintains an effective quality assurance and quality control program.

The VMDL has formal anatomical pathology, clinical pathology, and clinical microbiology training programs. The VMDL faculty hold academic appointments in the Departments of Veterinary Pathobiology and Veterinary Biomedical Sciences. The director is administratively responsible to the dean of the College of Veterinary Medicine for the overall operation of the laboratory and its multiple missions of public service, teaching, research and continuing education/extension.

The VMDL faculty and staff work diligently to provide relevant, timely and cost-effective diagnostic services to our clients and to create a positive learning environment for our students and residents. In 2024, veterinarians, animal owners and researchers from 103 Missouri counties and cities, as well as from 51 states and provinces outside Missouri, continued to rely on the Veterinary Medical Diagnostic Laboratory’s (VMDL) diagnostic services. The lab performed approximately 63,500 total cases and conducted nearly 204,500 tests.