MU Veterinary Medical Diagnostic Laboratory

  • Home
  • About
    • Mission Statement
    • AAVLD Accredited Lab
    • NAHLN Level 1 Lab
    • FDA Vet-LIRN Tier I Lab
    • NPIP Lab
    • Our Location
    • Quality Assurance System
      • Login to VMDL QA/QC Site
    • Policies
      • Basic VMDL Policies
      • Use of VMDL Necropsy Facility
  • Tests and Fees
    • Browse by Category
    • Avian Tests and Fees
    • Canine Tests and Fees
    • Equine Tests and Fees
    • Feline Tests and Fees
    • One Health-Rabies Laboratory
    • Porcine Tests and Fees
    • Ruminant Tests and Fees
    • Fee Guide (PDF)
  • Submission Forms
  • Lab Sections
    • Director’s Office
    • Anatomic Pathology
    • Avian Pathology
    • Bacteriology & Mycology
    • Clinical Pathology
    • Histopathology
    • Molecular Diagnostics
    • One Health-Rabies Laboratory
    • Serology
    • Analytical Chemistry and Toxicology
  • Accounting and Billing
  • Resources
  • FAQs

Search Results for: Equine Viral Arteritis SN

Equine Viral Arteritis SN

Lab Section Serology
Test Description Serum neutralization test for the detection of antibodies to the equine arteritis virus
Species Equine

SUBMISSION DETAILS

Sample Type Serum (1 ml)
Container Red top tube or other sterile container without additive
Special Submission Instructions
  • Centrifuge clotted blood and send serum only.
  • Ship with cold packs.
Turnaround time 4-7 business days

Equine Tests and Fees

Test Name Section Price
Abortion Panel Anatomic Pathology $200.00
Abortion Screen Bacteriology $51.00
ACTH Stimulation Clinical Pathology $55.00
Activated Partial Thromboplastin Time Clinical Pathology $27.00
Activated Partial Thromboplastin Time (APTT) / Prothrombin Time (PT) Combo Clinical Pathology $48.00
Aflatoxin M1 in Milk and Liver Toxicology $102.00
Alanine aminotransferase Clinical Pathology $11.00
Albumin Clinical Pathology $11.00
Alkaline phosphatase Clinical Pathology $11.00
Aerobic Bacterial Culture, Isolation and Identification (excluding blood samples) Bacteriology $31.00
Anaerobic Bacterial Culture Bacteriology $31.00
Aerobic and Anaerobic Combo up to 3 aerobic susceptibilities for 1 specimen
(Fixed price; must select at submission)
Bacteriology $130.00
Aerobic Bacterial Culture with up to 3
susceptibilities for 1 specimen
(Fixed price; must select at submission)
Bacteriology $125.00
Antimicrobial Susceptibility Test Bacteriology $34.00
Aspartate aminotransferase Clinical Pathology $11.00
Aspergillus AGID Serology $21.00
Baermann Sedimentation Clinical Pathology $24.00
Bicarbonate Clinical Pathology $11.00
Bile Post Clinical Pathology $27.00
Bile Pre or Single Clinical Pathology $27.00
Bilirubin, Direct Clinical Pathology $11.00
Bilirubin, total Clinical Pathology $11.00
Biopsy Anatomic Pathology $84.00
Blastomyces AGID Serology $21.00
Blastomyces and Histoplasma AGID Serology $37.00
Blood Culture Bacteriology $45.00
Blood Parasite Exam Clinical Pathology $23.00
Blood Urea Nitrogen Clinical Pathology $11.00
Bone Marrow Aspirate Clinical Pathology $55.00
Bone Marrow Core Biopsy Evaluation Clinical Pathology $84.00
Borrelia burgdorferi IFA Serology $26.00
Borrelia burgdorferi PCR Molecular Diagnostics $45.00
Bronchoalveolar Lavage Analysis Clinical Pathology $50.00
Buffy Coat Clinical Pathology $45.00
Calcium Tox Toxicology $30.00
Calcium Urine Clinical Pathology $14.00
Calcium Clinical Pathology $11.00
CBC Equine (includes slide review and fibrinogen) Clinical Pathology $37.00
CBC Large Animal (includes slide review and fibrinogen) Clinical Pathology $37.00
Chloride Urine Clinical Pathology $14.00
Chloride Clinical Pathology $11.00
Cholesterol Clinical Pathology $11.00
Coagulation Panel 2 plus Fibinogen Clinical Pathology $105.00
Coagulation Panel 1 Clinical Pathology $82.00
Coccidioides immitis AGID Serology $21.00
Coggins Test ELISA Serology $9.00
Comprehensive Blood Smear Exam Clinical Pathology $45.00
Contagious Equine Metritis Culture Bacteriology $35.00
Copper Toxicology $30.00
Cortisol Clinical Pathology $29.00
Cosmetic Necropsy Anatomic Pathology $225.00
Creatine Kinase Clinical Pathology $11.00
Creatinine Clinical Pathology $11.00
Creatinine-Urine Clinical Pathology $14.00
Cryptococcus neoformans Antibody Tube Agglutination Serology $21.00
Cryptococcus neoformans Antigen Latex Agglutination Serology $22.00
Cryptosporidia Exam Clinical Pathology $21.00
CSF Analysis Clinical Pathology $60.00
Cyanide (Prussic Acid) Toxicology $20.00
Cytological Examination Clinical Pathology $45.00
D-Dimer Clinical Pathology $49.00
Dexamethasone Suppression Test 2 samples Clinical Pathology $55.00
Dexamethasone Suppression Test 3 samples Clinical Pathology $76.00
Electrolyte and Mineral Profile Clinical Pathology $20.00
Electron Microscopy
Pool of 5 submitted together – $250.00
Virology $70.00
Endogenous ACTH Clinical Pathology $49.00
Equine Enteric Panel PCR Molecular Diagnostics $103.00
Equine Enteric Panel PLUS PCR Molecular Diagnostics $142.00
Equine Herpes 1 and 4 PCR Molecular Diagnostics $47.00
Equine Herpesvirus 1 Genotyping PCR Molecular Diagnostics $85.00
Equine Infectious Anemia (EIA) ELISA Serology $9.00
Equine Infectious Anemia (EIA) AGID Serology $9.50
Equine Maxi Panel Clinical Pathology $42.00
Equine Mini Panel Clinical Pathology $29.00
Equine Neonatal/Foal Enteric Panel PCR Molecular Diagnostics $108.00
Equine Neurological Panel PCR Molecular Diagnostics $89.00
Equine Respiratory Panel Molecular Diagnostics $80.00
Equine Rhinopneumonitis SN Serology $16.00
Equine Viral Arteritis SN Serology $21.00
Fecal Flotation Clinical Pathology $18.00
Fibrinogen Automated Clinical Pathology $33.00
Fibrinogen Heat Precipitation Method Clinical Pathology $12.00
Foal IgG Snap Test Clinical Pathology $38.00
Fungal Culture – Systemic Bacteriology $31.00
Gamma Glutamyltransferase Clinical Pathology $11.00
GC MS Screen Toxicology $153.00
GLDH Clinical Pathology $11.00
Glucose Urine Clinical Pathology $14.00
Glucose Clinical Pathology $11.00
Gross Necropsy Only (One animal, 50% off for each additional animal, up to three) Anatomic Pathology $88.00
Histoplasma capsulatum AGID Serology $21.00
ICP-OES (Metals and Metalloids in Liver and Kidney) Toxicology $45.00
ICP-OES (Metals in Serum and Plasma) Toxicology $45.00
ICP-OES (Selenium in Serum and Plasma) Toxicology $45.00
Immunohistochemistry Anatomic Pathology $40.00
Influenza A PCR Molecular Diagnostics $45.00
Insulin Glucose Clinical Pathology $36.00
Iron Toxicology $30.00
Joint Aspirate Pleural Fluid Peritoneal Fluid Culture Bacteriology $45.00
Large Animal Liver Profile Clinical Pathology $27.00
Large Animal Spinal Exam Anatomic Pathology $100.00
Lawsonia intracellularis PCR Molecular Diagnostics $39.00
Lead in Tissue Toxicology $30.00
Lead in Whole Blood Toxicology $30.00
Leptospirosis 6 Serovars Titer MAT Serology $18.00
Leptospira spp PCR Molecular Diagnostics $45.00
Magnesium Tox Toxicology $30.00
Magnesium Clinical Pathology $11.00
Fresh and Fixed Tissue Exam plus Lab Tests Anatomic Pathology $181.00
Mastitis Antimicrobial Susceptibility Test (up to 2 organisms) Bacteriology $33.50
Microscopic Examination of Specimen Bacteriology $16.00
Fecal Egg Count
(10+) $17.25 each
Clinical Pathology $20.00
Multiple Lymph Node Cytology Clinical Pathology $67.50
Multiple Synovial Cytology Clinical Pathology $67.50
Necropsy with Histopathology
(One animal, 50% off for each additional animal, up to three)
Anatomic Pathology $110.00
Necropsy with Histopathology and Lab Tests for One-Organ
(One animal, 50% off each additional animal, up to three)
Anatomic Pathology $200.00
Neorickettsia risticii PCR Molecular Diagnostics $45.00
Nitrate in Ocular Fluid Toxicology $42.00
Occult Blood Clinical Pathology $15.00
Ophthalmology Antimicrobial Susceptibility Panel – up to 2 organisms Bacteriology $37.00
Osmolality (measured) Urine Clinical Pathology $21.00
Osmolality measured Clinical Pathology $22.00
Peritoneal, Pleural, Pericardial Fluid Analysis Clinical Pathology $55.00
pH of Rumen Contents Toxicology $13.00
Phenobarbital Level Clinical Pathology $30.00
Phosphorus Urine Clinical Pathology $14.00
Phosphorus, inorganic Clinical Pathology $11.00
Poisonous Plant and Animal Identification Toxicology $27.00
Potassium Urine Clinical Pathology $14.00
Potassium Clinical Pathology $11.00
Potomac Horse Fever PCR Molecular Diagnostics $45.00
Progesterone Clinical Pathology $21.00
Protein Creatinine Ratio Urine Clinical Pathology $24.00
Protein, Total Clinical Pathology $11.00
Prothrombin Time Clinical Pathology $27.00
Rabies Test Anatomic Pathology $37.00
Renal Panel Clinical Pathology $27.00
Rotavirus Group A PCR Molecular Diagnostics $45.00
Salmonella Culture Bacteriology $31.00
Salmonella PCR Molecular Diagnostics $38.00
Sample Preparation Bacteriology $25.00
Sodium (urine) Clinical Pathology $14.00
Sodium Clinical Pathology $11.00
Streptococcus equi subsp. equi PCR Molecular Diagnostics $45.00
Synovial Fluid Analysis Clinical Pathology $55.00
Tick Panel IgG IFA Serology $86.50
Tick Panel PCR Molecular Diagnostics $99.00
Toxoplasma gondii PCR Molecular Diagnostics $45.00
Triglyceride Clinical Pathology $11.00
Urea nitrogen Urine Clinical Pathology $14.00
Uric Acid Clinical Pathology $11.00
Urinalysis Complete Clinical Pathology $26.00
Vesicular Stomatitis Indiana Virus Serology $15.00
Vesicular Stomatitis New Jersey Virus Serology $15.00
Virus Isolation Virology $40.00
Vitamin E Toxicology $30.00
West Nile Virus IgM ELISA Serology $30.00
West Nile Virus PCR Molecular Diagnostics $45.00
Yeast Antimicrobial Susceptibility Test Bacteriology $61.00
Zinc Toxicology $30.00

Tests Performed by Serology

Anaplasma marginale ELISA
(10+) $9.75 each / (50+) $7.00 each
$12.50
Anaplasma phagocytophilia IFA $29.00
Aspergillus AGID $21.00
Blastomyces AGID $21.00
Blastomyces and Histoplasma AGID $37.00
Bluetongue ELISA
(10+) $7.25 each
$9.00
Borrelia burgdorferi (Lyme Disease) Titer IFA $26.00
Bovine Leukemia (Leukosis) Virus ELISA
(10+) $8.50 each / (50+) $7.00 each
$9.50
Bovine Respiratory Syncytial Virus (BRSV) SN
(10+) $7.75 each / (50+) $7.25 each
$9.50
Bovine Viral Diarrhea (BVD) Antigen Detection ELISA
(10+) $7.75 each
$10.00
Bovine Viral Diarrhea (BVD) Virus Type 1 SN
(10+) $8.50 each
$10.50
Bovine Viral Diarrhea (BVD) Virus Type 2 SN
(10+) $8.50 each
$10.50
Brucella abortus/suis Card $5.00
Brucella abortus/suis Card and Pseudorabies Virus (PRV) ELISA $10.50
Brucella Rapid Ab Test (Canine Brucellosis) $18.50
CAE and OPP Detection (Small Ruminant Lentivirus) ELISA
(10+) $8.25 each / (30+) $7.50 each
$12.00
Canine Distemper IgG Titer IFA $27.00
Canine Distemper IgM Titer IFA $27.00
Canine Distemper/Parvo Titer ELISA $26.00
Canine Parvovirus IgG IFA $26.00
Canine Parvovirus IgM IFA $26.00
Chronic Wasting Disease (CWD) ELISA $24.00
Coccidioides immitis AGID $21.00
Cryptococcus neoformans Antibody Tube Agglutination $21.00
Cryptococcus neoformans Antigen Latex Agglutination $22.00
Cryptosporidium parvum and Giardia FA $28.00
Ehrlichia canis IFA $29.00
Epizootic Hemorrhagic Disease Virus (EHDV) AGID
(10+) $10.00 each / (50+) $7.50 each
$12.50
Equine Infectious Anemia (EIA) AGID $9.50
Equine Infectious Anemia (EIA) ELISA $9.00
Equine Rhinopneumonitis (Herpesvirus) SN $16.00
Equine Viral Arteritis SN $21.00
Feline Immunodeficiency Virus (FIV), Feline Leukemia Virus (FeLV)
and Dirofilaria immitis 3 Way Snap Test
$41.00
Feline Infectious Peritonitis IFA $27.00
Feline Leukemia Virus IFA $28.75
Heartworm Antigen ELISA $17.00
Histoplasma capsulatum AGID $21.00
Infectious Bovine Rhinotracheitis (IBR) Virus SN
(10+) $8.25 each
$9.75
Johne’s Disease ELISA
(10+) $8.50 each / (50+) $6.00 each
$10.00
Leptospirosis 6 Serovars Titer MAT $18.00
Mycoplasma hyopneumoniae ELISA
(10+) $7.25 each / (50+) $6.75 each
$8.00
Neospora caninum ELISA
(10+) $7.50 each / (50+) $6.75 each
$8.50
Parainfluenza 3 (PI3) Virus SN
(10+) $7.50 each / (50+) $6.50 each
$9.50
Porcine Reproductive and Respiratory Syndrome Virus (PRRSV) ELISA
(10+) $7.00 each / (50+) $6.00 each
$8.00
Pseudorabies (PRV) ELISA
(10+) $5.75 each / (50+) $5.00 each
$6.75
Rickettsia rickettsii (Rocky Mountain Spotted Fever) IFA $26.00
Swine Influenza ELISA
(10+) $8.00 each / (50+) $6.75 each
$15.00
Systemic Fungal Panel AGID $60.00
Tick Panel IgG IFA $86.50
Toxoplasma gondii IgG ELISA $13.00
Vesicular Stomatitis Virus Indiana SN $15.00
Vesicular Stomatitis Virus New Jersey SN $15.00
West Nile Virus IgM ELISA $30.00

All Tests C through E

Tests: A-B | C-E | F-M | N-S | T-Z

Click on test name for details.

Test Name Section Species Price
CAE and OPP Detection (Small Ruminant Lentivirus) ELISA
(10+) $8.25 each/ (30+) $7.50 each
Serology Caprine, Ovine $12.00
Calcium Tox Toxicology Avian, Canine, Equine, Feline, Porcine, Ruminant $30.00
Calcium – Urine Clinical Pathology Canine, Equine, Feline, Porcine, Ruminant $14.00
Calcium Clinical Pathology Avian, Canine, Equine, Feline, Porcine, Ruminant $11.00
Canine Adenovirus type 2 PCR Molecular Diagnostics Canine $45.00
Canine Coronavirus PCR Molecular Diagnostics Canine $45.00
Canine Enteric Panel PCR Molecular Diagnostics Canine $105.00
Canine Distemper IgG IFA Serology Canine $27.00
Canine Distemper IgM IFA Serology Canine $27.00
Canine Distempter/ Parvo Titer ELISA Serology Canine $26.00
Canine Distemper Virus PCR Molecular Diagnostics Canine $45.00
Canine Herpesvirus PCR Molecular Diagnostics Canine $45.00
Canine Parvovirus IgG IFA Serology Canine $26.00
Canine Parvovirus IgM IFA Serology Canine $26.00
Canine Respiratory Panel PCR Molecular Diagnostics Canine $97.00
CBC Equine Clinical Pathology Equine $37.00
CBC Large Animal (includes slide review and fibrinogen) Clinical Pathology Equine, Ruminant $37.00
CBC Small Animal (includes retic and slide review) Clinical Pathology Canine, Feline $33.00
Chlamydia/Chlamydophila PCR Molecular Diagnostics Feline, Ruminant $45.00
Chloride – Urine Clinical Pathology Canine, Equine, Feline, Porcine, Ruminant $14.00
Chloride Clinical Pathology Avian, Canine, Equine, Feline, Porcine, Ruminant $11.00
Cholesterol Clinical Pathology Avian, Canine, Equine, Feline, Porcine, Ruminant $11.00
Chronic Wasting Disease ELISA Serology Cervid $24.00
Chronic Wasting Disease (CWD)
Immunohistochemistry (IHC)
(Both Sites)
Anatomic Pathology Cervid $45.00
Coagulation Panel 2 + Fibrinogen Clinical Pathology Canine, Equine, Feline $105.00
Coagulation Panel 1 Clinical Pathology Canine, Equine, Feline $82.00
Coccidioides immitis AGID Serology Canine, Equine, Feline, Porcine, Ruminant $21.00
Coggins Test ELISA Serology Equine $9.00
Comprehensive Blood Smear Exam Clinical Pathology Avian, Canine, Equine, Feline, Porcine, Ruminant $45.00
Contagious Equine Metritis Culture Bacteriology Equine $35.00
Coombs Test Direct Clinical Pathology Canine $40.00
Copper Toxicology Avian, Canine, Equine, Feline, Porcine, Ruminant $30.00
Cortisol Clinical Pathology Avian, Canine, Equine, Feline, Porcine, Ruminant $29.00
Cosmetic Necropsy Anatomic Pathology Avian, Canine, Equine, Feline, Porcine, Ruminant $225.00
Creatine Kinase Clinical Pathology Avian, Canine, Equine, Feline, Porcine, Ruminant $11.00
Creatinine Clinical Pathology Avian, Canine, Equine, Feline, Porcine, Ruminant $11.00
Creatinine (Creat)- Urine Clinical Pathology Avian, Canine, Equine, Feline, Porcine, Ruminant $14.00
Cryptococcus neoformans Antibody Tube Agglutination Serology Avian, Canine, Equine, Feline, Ruminant $21.00
Cryptococcus neoformans Antigen Latex Agglutination Serology Avian, Canine, Equine, Feline, Ruminant $22.00
Cryptosporidia Exam Clinical Pathology Avian, Canine, Equine, Feline, Porcine, Ruminant $21.00
Cryptosporidium parvum and Giardia FA Serology Canine, Feline, Ruminant $28.00
CSF Analysis Clinical Pathology Avian, Canine, Equine, Feline, Porcine, Ruminant $60.00
Cyanide (Prussic Acid) Toxicology Avian, Canine, Equine, Feline, Porcine, Ruminant $20.00
Cytological Examination Clinical Pathology Avian, Canine, Equine, Feline, Porcine, Ruminant $45.00
D-Dimer Clinical Pathology Canine, Equine, Feline $49.00
Dexamethasone Suppression
Test (2 samples)
Clinical Pathology Avian, Canine, Equine, Feline, Porcine, Ruminant $55.00
Dexamethasone Suppression
Test (3 samples)
Clinical Pathology Avian, Canine, Equine, Feline, Porcine, Ruminant $76.00
Ehrlichia canis IFA Serology Canine $29.00
Ehrlichia spp PCR Molecular Diagnostics Canine $39.00
Electrolyte and Mineral Profile Clinical Pathology Avian, Canine, Equine, Feline, Porcine, Ruminant $20.00
Electron Microscopy
Pool of 5 submitted together – $250.00
Virology Avian, Canine, Equine, Feline, Porcine, Ruminant $70.00
Endogenous ACTH Clinical Pathology Equine $49.00
Enteric Screen Bacteriology Canine, Porcine, Ruminant $62.00
Epizootic Hemorrhagic Disease Virus AGID
(10+) $10.00 each / (50+) $7.50 each
Serology Ruminant $12.50
Epizootic Hemorrhagic Disease Virus PCR
(3+)  $37.00 each / (10+) $35.00 each
Molecular Diagnostics Ruminant $39.00
Equine Enteric Panel PCR Molecular Diagnostics Equine $103.00
Equine Enteric Panel PLUS PCR Molecular Diagnostics Equine $142.00
Equine Herpesvirus 1 Genotyping PCR Molecular Diagnostics Equine $85.00
Equine Herpes 1 and 4 PCR Molecular Diagnostics Equine $47.00
Equine Infectious Anemia (EIA) ELISA Serology Equine $9.00
Equine Infectious Anemia (EIA) AGID Serology Equine $9.50
Equine Maxi Panel Clinical Pathology Equine $42.00
Equine Mini Panel Clinical Pathology Equine $29.00
Equine Neonatal/Foal Enteric Panel PCR Molecular Diagnostics Equine $108.00
Equine Neurological Panel PCR Molecular Diagnostics Equine $89.00
Equine Rhinopneumonitis SN Serology Equine $16.00
Equine Viral Arteritis SN Serology Equine $21.00
Ergot Alkaoids in Feedstuff
(10+) $100.00 each
Toxicology $120.00
Ergot Fescue Alkaloids in Feedstuff
(10+) $120.00 each
Toxicology $150.00

Under the Scope – Summer 2021

New VMDL Fee Guide Available! Pricing Effective July 1

Histopathology/Biopsy Changes:

  • Our base biopsy price ($72.50) will now include evaluation of up to three sites from the same animal. Additional sites from the same animal will be charged an additional $30 per site.
  • A dissection fee ($30) will be assessed for large surgical biopsies such as amputated limbs, whole spleens, mandible/maxilla specimens, whole mammary chains, digits, and very large masses. These cases require a significant amount of additional pathologist time to locate the lesion(s) and collect appropriate sections.
  • A new test code has been created for formalin fixed tissues derived from an offsite necropsy examination. This test is called “Fixed Tissue Exam- from necropsy” and there is no limit on the number of fixed tissues/organs that can be submitted. The cost for this evaluation is $100 and may not be used for tissue excised from a live animal during a surgical procedure.

Disposal Changes (with necropsy only):

  • Disposal/Incineration fees now apply for communal cremation of avian and companion animals, as well as food animals weighing 500 pounds or less. Please see page 4 for details.

Tests Added:

  • Equine Neonatal/Foal Enteric PCR Panel: Rotavirus A PCR, Salmonella PCR, C. difficile toxin A&B and C. perfringes toxin ELISA ($90)

Tests Discontinued:

  • Feline Heartworm Antibody Lateral Flow Immunoassay
  • Equine Viral Arteritis ELISA

If you have any questions about our new fee guide, please feel free to call the laboratory at 573-882-6811. We strive to provide high quality diagnostics at a reasonable price.


Bone Biopsy: Sampling for Success

Using histopathology to definitively diagnose canine bone lesions is very important, since benign and neoplastic osteodestructive processes can be indistinguishable on radiographs and diagnosis greatly affects the treatment regimen. However, representative sampling of the lesion by bone biopsies (e.g., Jamshidi bone needle, Michele trephine) can be quite challenging. The center of the lesion is often necrotic, while the edges may simply be reactive changes of bone and periosteum. Sometimes, despite everyone’s best efforts, the tissue sampled is non-diagnostic. A retrospective study of VMDL cases demonstrates that neoplastic changes were not observed in 20 out of 61 (33 percent) primary bone tumor cases in dogs at the first bone-core biopsy attempt (Kuroki et al. Accuracy of bone-core biopsy for diagnosis of primary bone tumors in dogs. 2012 ACVP and ASVCP annual meeting. Paper #D-47). While it’s a good idea to prepare your clients for the possibility of an unsuccessful diagnosis, here are a few things you can do to improve your chances of getting a definitive diagnosis:

  • Sample multiple areas of the lesion! In our retrospective study one or two biopsy sample(s) yielded approximately 60 percent positive diagnosis while three or more biopsy samples yielded 93 percent positive diagnosis. If possible, it’s best to sample the radiographic center of the lesion as well as areas of sclerosis on both sides.
  • Use imaging to plan and confirm biopsy sites.
  • Provide a detailed history with the specimens, ideally including an image or diagram showing the number and location of the biopsy sites.

Possible complications associated with bone-core biopsies include bone fracture, nerve or blood vessel damage and infection. If an amputation is performed, clinicians may submit the entire fresh limb to the VMDL for adequate sampling and histopathology examination. Please feel free to call the laboratory with questions regarding bone biopsy or whole limb submission.


Bacillary Hemoglobinuria in Missouri

By: Rosalie Ierardi, DVM

Photo credit: Renata Mammone, DVM

By now, many of you have heard about a recent cluster of bacillary hemoglobinuria cases in north-central Missouri. At least seven cattle between December 2020 and May 2021 met the criteria of hemoglobinuria, hepatic necrosis, and identification of Clostridium novyi/Clostridium haemolyticum in the liver lesions. In five of these cases, deer flukes and/or fluke eggs were identified in the liver. Seven cattle may not sound like much, until one considers that during the previous decade (2010-2020) only three suspected cases of clostridial hepatitis were received by the VMDL. We are continuing to investigate this group of cases, and diagnostic submissions are highly encouraged.

In any discussion of bacillary hemoglobinuria, it’s worthwhile to consider two closely related conditions with a very similar pathogenesis: bacillary hemoglobinuria, “red water disease” versus infectious necrotic hepatitis, “black disease”.

Bacillary hemoglobinuria (BH) is caused by Clostridium haemolyticum, which produces a toxin that causes intravascular hemolysis and acute hepatic necrosis. The hemolysis causes severe anemia, and the disease is almost invariably fatal.

Sudden death is frequent. Clinical signs include depression, anorexia and fever. Hemoglobinuria is the hallmark. At necropsy, the most consistent and characteristic lesion is a focally extensive area of hepatic necrosis, often surrounded by a dark red halo.

Infectious necrotic hepatitis (INH) is a similar condition which occurs commonly in sheep, occasionally in cattle, and rarely in horses. INH is caused by Clostridium novyi type B. Liver lesions are fairly similar to those in BH, but hemoglobinuria is absent. Like BH, this disease is nearly always fatal.

Both C. haemolyticum and C. novyi type B live in soil as highly resistant spores. Once ingested by grazing animals, spores can lay dormant in the liver for months. Disease occurs only when liver injury creates the anaerobic conditions required for bacterial growth. This is typically attributed to migration of Fasciola hepatica (common liver fluke), but bacillary hemoglobinuria is also reported in cattle without evidence of liver flukes. While Fasciola hepatica is thought to be the most common predisposing factor for BH, this species is seldom reported in Missouri. Missouri cattle are more likely to be infected with Fascioloides magna, the deer fluke. The relationship between F. magna and BH is less thoroughly studied.

F. hepatica and F. magna have similar and intricate life cycles. Development of the flukes depends on availability of snail intermediate hosts, a moist environment, and temperatures above 50 degrees Fahrenheit. Fluke eggs are deposited in the feces of cattle and sheep (F. hepatica) or deer (F. magna). Eggs hatch and develop through several life stages, mostly within snails, until they mature into the infective larval stage known as metacercariae. Metacercariae encyst on vegetation, awaiting ingestion by the mammalian host. In favorable conditions, eggs and/or metacercariae can survive on pastures for months.

Once ingested, F. hepatica migrates to the liver and eventually settles in the bile ducts, where its eggs are passed into the feces. In the case of F. magna, cattle and sheep are dead-end hosts. The flukes wander aimlessly in the liver and cause considerable damage until they are walled off with fibrous connective tissue by the host. The eggs never make it into the feces.

When examining a bovine carcass with hemoglobinuria and/or icterus, examine the liver carefully. Necrotic lesions are sometimes subtle. Migration tracts of F. magna within the liver typically contain black pigment. It’s best to submit multiple small formalin-fixed samples for histopathology and several large fresh samples for bacterial culture. Laboratory confirmation can be challenging, as C. haemolyticum and C. novyi type B are difficult to isolate. Diagnosis is supported by fluorescent antibody testing (FAT), which is routinely performed at the VMDL. Ideally, carcasses should be burned, buried, or removed from the premises to reduce contamination of soil.

Vaccination is the best way to protect cattle from BH and INH. To prevent bacillary hemoglobinuria, the vaccine must be labeled for protection against C. haemolyticum. Most “seven-way” multivalent vaccines do not protect against BH, but C. haemolyticum is usually included in eight-way or nine-way products. Duration of immunity is only about six months; in especially high-risk situations, biannual vaccination may be warranted. Although avoidance of liver flukes is an attractive target for disease control, this is often an impractical approach.

Previous page

Client Portal - View Lab Results

Client Supplies Order Form

Lab Sections

  • Anatomic Pathology
  • Avian Pathology
  • Bacteriology & Mycology
  • Clinical Pathology
  • Histopathology
  • Serology
  • Analytical Chemistry and Toxicology
  • Molecular Diagnostics

Message from the Director

The University of Missouri Veterinary Medical Diagnostic Laboratory (VMDL) is a full-service veterinary diagnostic laboratory and Missouri’s only laboratory accredited by the American Association of Veterinary Laboratory Diagnosticians. The VMDL is a Level I Lab of the National Animal Health Laboratory Network, Tier I Lab of the FDA Veterinary Laboratory Response Network, and a National Poultry Improvement Plan authorized lab. The VMDL maintains an effective quality assurance and quality control program.

The VMDL has formal anatomical pathology, clinical pathology, and clinical microbiology training programs. The VMDL faculty hold academic appointments in the Departments of Veterinary Pathobiology and Veterinary Biomedical Sciences. The director is administratively responsible to the dean of the College of Veterinary Medicine for the overall operation of the laboratory and its multiple missions of public service, teaching, research and continuing education/extension.

The VMDL faculty and staff work diligently to provide relevant, timely and cost-effective diagnostic services to our clients and to create a positive learning environment for our students and residents. In 2024, veterinarians, animal owners and researchers from 103 Missouri counties and cities, as well as from 51 states and provinces outside Missouri, continued to rely on the Veterinary Medical Diagnostic Laboratory’s (VMDL) diagnostic services. The lab performed approximately 63,500 total cases and conducted nearly 204,500 tests.

About the MU VMDL

The VMDL is a full-service laboratory accredited by the American Association of Veterinary Laboratory Diagnosticians (AAVLD). The VMDL provides in-depth laboratory diagnostic support to veterinary practitioners, livestock and poultry industry interests, companion animal interests, wildlife conservationists, scientists utilizing animals in their research throughout the university, state and regulatory officials, and clinicians of the MU Veterinary Health Center.

Our Location

Click to open a larger map

901 E. Campus Loop
Columbia, MO 65211

VMDL News

  • VMDL Newsletter
  • Bovine Diagnostic Summaries

VMDL Annual Reports

  • 2024 Annual Report
  • 2023 Annual Report
  • 2022 Annual Report
  • 2021 Annual Report
  • Annual Report Archive

VMDL Employment

  • Faculty Employment
  • Staff Openings
  • Residencies
  • Pathology Externship

Contact

  • Contact the VMDL
  • CVM Director of Communications
  • CVM Webmaster

Links

  • College of Veterinary Medicine
  • Biomedical Sciences
  • Veterinary Pathobiology
  • Veterinary Medicine and Surgery
  • Veterinary Health Center (VHC)
  • VHC Wentzville

© 2025 — Curators of the University of Missouri. All rights reserved. DMCA and other copyright information. An equal opportunity/access/affirmative action/pro-disabled and veteran employer. Disability & Accessibility information